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  • WJPPS JUNE ISSUE PUBLISHED
  • JUNE 2018 Issue has been successfully launched on 1 June 2018

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  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from 6.647 to 7.421 due to high quality Publication at International Level

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Abstract

ISOLATION, PURIFICATION AND OPTIMIZATION OF CHITOSANASE FROM ASPERGILLUS FUMIGATUS ISOLATED FROM AGRICULTURE FIELD, MAHABUBANAGAR, TELANGANA

P. Shivakumar Singh and G. M. Vidyasagar*

ABSTRACT

Objective: Isolation, purification, optimization of chitosanase production using a common fungi Aspergillus fumigatus. Methods: The standard methods followed like Collection of soil sample, Screening of microbes in chitosanase detection broth, Optimization of Physical Parameters, Optimization of Nutritional Parameters and SDS-PAGE were recorded. Results: Chitosanases have inward much consideration as of their extensive series of applications. Even though the majority fungal chitosanases use sugar as their chief carbon source, the present investigation represents the chitosanase production using a common fungi Aspergillus fumigatus. The isolation, purification methods were standardised. The optimization of physical parameters like pH, temperature, inoculum size, agitation speed and optimization of nutritional parameters like carbon source, nitrogen source were reported. Conclusion: This broad range of specificity is advantageous for the production of partially deacetylated chitosan oligosaccharides and for the economical use of chitosan.

Keywords: Isolation, optimization, chitosanase production, fungi of Mahabubnagar District. Telangana State.


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