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  • WJPPS SEPTEMBER ISSUE PUBLISHED
  • SEPTEMBER 2017 Issue has been successfully launched on 1 September 2017

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  • WJPPS Rank with Index Copernicus Value 52.51 due to high reputation at International Level

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  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from 6.041 to 6.647 due to high quality Publication at International Level

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Abstract

ISOLATION, PURIFICATION AND OPTIMIZATION OF CHITOSANASE PRODUCTION FROM A COMMON MAHABUBNAGAR AGRICULTURAL FIELD FUNGI ASPERGILLUS FUMIGATUS OF TELANGANA STATE

P. Shivakumar Singh and G. M. Vidyasagar*

ABSTRACT

Objective: Isolation, purification, optimization of chitosanase production using a common fungi Aspergillus fumigatus. Methods: The standard methods followed like Collection of soil sample, Screening of microbes in chitosanase detection broth, Optimization of Physical Parameters, Optimization of Nutritional Parameters and SDS-PAGE were recorded. Results: Chitosanases have inward much consideration as of their extensive series of applications. Even though the majority fungal chitosanases use sugar as their chief carbon source, the present investigation represents the chitosanase production using a common fungi Aspergillus fumigatus. The isolation, purification methods were standardised. The optimization of physical parameters like pH, temperature, inoculum size, agitation speed and optimization of nutritional parameters like carbon source, nitrogen source were reported. Conclusion: This broad range of specificity is advantageous for the production of partially deacetylated chitosan oligosaccharides and for the economical use of chitosan.

Keywords: Isolation, optimization, chitosanase production, fungi of Mahabubnagar District. Telangana State.


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