MOLECULAR PHYLOGENY OF PTEROCARPUS MARSUPIUM AND PTEROCARPUS SANTALINUS
*T.B.V.Lakshmi, K.Sriramamurthy
ABSTRACT
Pterocarpus santalinus and Pterocarpus marsupium are the
endangered perennial species listed in the Red data book. Pterocarpus
santalinus or Red Sanders is native to India, medicinally important in
treating uterine bleeding and heartwood of the bark used to treat
inflammation. Pterocarpus marsupium or the Indian Kino or the
Malabar Kino Tree or the Kino Tree is a medium to large, deciduous
tree native to India, Nepal, and Srilanka.This indicates the need for
preservation and conservation efforts at the population level. In this
randomly amplified polymorphic DNA (RAPD) technique was
employed for the molecular characterization and Barcoding was used
for molecular identification of Pterocarpus santalinus and Pterocarpus
marsupium. Twenty two decamer oligonucleotide primers were used to amplify the genomic
DNA which was isolated from the leaf samples. Out of twenty two primers used, five did not
amplify, seven gave faint and non-reproducible bands, while ten gave species-specific
reproducible unique bands. The unique bands obtained in PCR amplification clearly
differentiated both the pterocarpus species from one another. Further study of genetic
variability with rbcL markers gave the molecular taxonomical difference in these two
Pterocarpus species.
Keywords: RAPD analysis, Barcoding, Pterocarpus santalinus, Pterocarpus marsupium, PCR, electrophoresis, universal primers.
[Download Article]
[Download Certifiate]