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Abstract

IN-VITRO METHODS OF SCREENING OF ANTI-CANCER AGENT

Shumaila Siddiqui*, Vineeta V. Khanvilkar and Deepali Jagdale

ABSTRACT

Global cancer population is more than 6.75 billion, and today cancer is the leading cause of mortality. Medical therapy in cancer treatment is an area of investigation for which efforts are being made to come up with novel chemical entities for better treatment of cancer. Discovery of new anti-cancer drugs is based on a mechanistic approach and has reliance on empiric cell-based screening of new drugs for its anti-cancer effects. To summarize the data related to current in-vitro methods for screening of anticancer agents. Reviewed here are some selected In-vitro cell based methods for screening of anti-cancer drugs. These are the combinations of systematic methods based on existing theories and models. New anti-cancer drug is necessary with novel profile of anti-tumor activity. This review will summarize most popular in-vitro cell based screening methods, which are economically good enough to establish the anti-cancer activity of newly evolved anti-cancer drugs.

Keywords: In-Vitro Screening Methods, Cell Based Assay, Anti-Cancer Agent, Tetrazolium Salt, Colorimetric Assay, DNA Fragmentation.


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