ANTI-FILARIAL EFFECT OF ANTIBIOTICS AGAINST PARASITE SETARIA CERVI: ROLE OF OXIDATIVE STRESS
D. Madkoriya, KN Sahare*, RK Patidar, M. Khare, and V. Singh
ABSTRACT
World health organization highlighted the requirement for
development of new drugs for filariasis. In the present study antifilarial
screening was carried out against Setaria cervi adult filarial with
varying concentrations of Tetracycline, Rifampicin and Doxycycline (2
- 20 mg/ml) individually to find out any involvement of oxidative
mechanism in the anti-filarial effect of these antibiotics in vitro.
Reduction in motility of the adult was measured after 24h in terms of
MTT reduction assay and correlated with the levels of MDA and nitric
oxide. Significant reduction in motility was recorded with increasing
concentration of Tetracycline and Rifampicin but with Doxycycline the
effect was not marked. Antibiotics with high antifilarial activity
revealed significant association with oxidative stress parameters in a dose dependent manner.
The results suggest that oxidative stress might be exploited to design and development of
novel antifilarial drug candidate.
Keywords: Antibiotics, Anti-filarial, Oxidative stress, Drug designing.
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