Abstract

GENETIC DIVERSITY ASSESSED BY RAPD MARKERS IN TOMATO CULTIVARS

Aina Yadav* and Dr. Neeraj Tandan

ABSTRACT

RAPD technique was used as a tool for assessing genetic diversity and relationships among 19 cultivars of tomato. 20 decamer primers were used for amplification and all of them showed good discriminatory power. A total of 129 bands were observed among the cultivars of tomato through 20 RAPD primers, of which 96 bands were polymorphic showing high range of variability (72.63% polymorphism) and 31 bands were monomorphic. On average the total number of bands generated per primer was 6.45, of which 4.8 were polymorphic and 1.55 were monomorphic. The values of genetic similarity ranged from 0.60-0.91, which indicate the substantial diversity (60-91%) among cultivars. The dendrogram constructed based on phylogenetic relationship analysis revealed that the hieghest genetic diversity (91.5%) found between (PDT-3-1-1 & PB Keshri) and (IIHR-1 & DVRT-1) whereas the lowest genetic diversity (60.5%) found between EC-538155 & EC-538423. Resulting dendrogram classified the 19 tomato cultivars into 6 distinct clusters. This study provides essential information that can be used to improve the tomato production, conservation and could be useful in gene mapping, marker assisted breeding and other genetic analysis for future tomato breeding programs.

Keywords: Tomato, RAPD, PCR, Polymorphism, cluster analysis and Genetic diversity.