Abstract

HEPATOPROTECTIVE ACTIVITY OF BETULA UTILIS BARK ON D-GALACTOSAMINE INDUCED HEPATIC INSULT

*B. Duraiswamy, M. N. Satishkumar, S. Gupta, M. Rawat, Om Porwal, R. Murugan

ABSTRACT

Objective: Betula utilis extracts was evaluated for In vitro and In vivo hepatoprotective activity against D-galactosamine induced hepatic damage. Material and methods: Betula utilis (family Betulaceae) bark was successively extracted with ethanol and aqueous to prepare extract of the plant. The in vitro isolated rat hepatocytes, hepatoprotective activity of ethanolic betula utilis (BUE) and aqueous betula utilis (BUA) extract was assessed against D-galactosamine (D-galN) at different concentration. Then in vivo hepatoprotective of BUE and BUA at dose 100 and 200 mg/kg body weight was carried out against D-galN, which on dose of 400 mg/kg body weight i.p.induces liver injury in rats. Results: The phytochemical screening on Betula utilis revealed the presence of primary metabolites such as, carbohydrates and protein, the secondary metabolites such as alkaloids, glycosides, saponins, sterols, flavonoids, phenolic compounds and tannins. The hepatocytes challenged with 50 μl of 10mM solution of D-galN. The group to which toxicant was added showed a significant increased in the ASAT, ALAT, and ALP enzyme level when compared to the normal. All the biochemical parameters were restored to normal significantly. Minimum restoration was observed at 62.5 g/ml in both the plant extract. Moreover in-vivo treatment with the BUE and BUA extract showed significant decreases in serum levels of ASAT, ALAT, ALP, LDH, and TB and a significant elevation in serum levels of the TP, TGL, and albumin in dose dependant manner. However, the normal architecture of the liver completely lost and marked centrilobular necrosis, focal necrosis and bile duct proliferation were observed in liver of DgalN treated group. It virtues further work towards the isolation of phytoconstituents from this plant Conclusions: These findings justify that Betula utilis extract protects the liver from severe damage caused by D-galN and may serve as a useful adjuvant in several clinical conditions associated with liver damage.

Keywords: Betula utilis, Hepatoprotective, D-galactosamine.