Abstract

TO STUDY THE EFFECT OF ANHYDROUS SOLVENT ON METHOTREXATE BY USING UV-SPECTROPHOTOMETER

Mahesh Pratapwar*, Dattatray Sabane, Atish Khilari, Vishal Jadhav and Mukesh Mohite

ABSTRACT

Three simple, precise and economical methods for UV have been developed for determination of Methotrexate in bulk formulation. Method A involves measurement of UV absorbance in Zero order derivative and Method B involves first order derivative are at 258 and 250 nm respectively. Method C deals with Area Under Curve measurement, which involves the calculation of integrated value of absorbance with wavelength range between 249-267 nm. The drug follows Beer-Lambert’s law in the concentration range of 10-50 μg/ml in all three methods. Results of analysis were validated statistically and found to be satisfactory. Thus proposed anhydrous solvent i.e. Sodium Carbonate and methods can be successfully applied for estimation of Methotrexate in routine analytical work.

Keywords: Methotrexate, Sodium Carbonate, Zero Order derivative, First order derivative, Area Under Curve method (AUC), UV- spectrophotometer.