Abstract

PROTECTIVE EFFECT OF D-LIMONENE ON ALUMINIUM CHLORIDE INDUCED MEMORY LOSS AND LEARNING DEFICIT IN MICE

M. Sarika*, A. Lalitha Devi, Rubeena Begum and Afreen Begum

ABSTRACT

Introduction: Various studies suggest that free radicals play a key role in Memory and Learning impairment in various cognitive disorders. D-Limonene is a monoterpene reported to possess anti-inflammatory and anti-oxidant property. Objective: Evaluation of protective effect of D-Limonene on Aluminium chloride induced Memory loss and Learning deficit in mice. Methodology: Albino mice (20-35g) were divided into 5 groups (n=6); Group 1- received water and standard pellet feed, Group 2- received Aluminium chloride (50mg/kg, p.o.), Group 3- received Rivastigmine (5mg/kg, p.o.) + Aluminium chloride. Groups 4 & 5- received Aluminium chloride + D-Limonene (2% & 4% w/w supplement enriched with pellet feed respectively). All the groups received respective treatment for 42 days. All the behavioural studies were performed on 21st & 42nd day. On 43rd day the brain was isolated for biochemical and Histopathological evaluation. Results: Administration of Aluminium chloride significantly induced Memory loss and learning deficit by decreased latency of fall, increases transfer latency and retention latency time. Altered levels of MDA, acetyl cholinesterase, nitric oxide in brain. There were significant changes in histoarchitecture of Hippocampus and Cerebral hemispheres when compared with normal control. Treatment with D-Limonene there is a dose dependent decrease in the oxidative Stress, Cognitive disorders and Neuronal degeneration. Conclusion: The present study suggests that protective effect of D-Limonene on aluminium chloride induced Memory and learning deficit, due to its anti-oxidants property.

Keywords: Various Altered levels of MDA, acetyl cholinesterase, nitric oxide in brain.