Abstract

FORMULATION AND EVALUATION OF BOSENTAN NANOSUSPENSIONS BY VARIOUS METHODS

Nagendra Reddy Janga*, Jagan Mohan Reddy Nallamilli, Narendra Devanaboyina

ABSTRACT

In the present study,an attempt was made to formulate and evaluate Bosentan Nanosuspension by various techniques. Nanosuspension containing the drug were prepared by two different techniques (Nanoprecipitation & Emulsification solvent diffusion method) using combinations of polymers such as Sodium lauryl sulphate (SLS), TWEEN-80, Poloxamer 188, Urea, Ethyl acetate and methanol). The Oral Nanosuspension were evaluated for various physical and biological parameters, drug content uniformity, particle size analysis, zeta potential, in-vitro drug release, short-term stability, drug- excipient interactions (FTIR). IR spectroscopic studies indicated that there are no drug-excipient interactions. Of all the formulations (PF1 – PF6 & SF1-SF6) PF3 formulation containing SLS 1.25%, Tween-80 0.2% and Poloxamer 1% were found to be promising which showed 99.55±0.84% release at the end of 30min & it follows first order drug release kinetics. For optimized formulation (PF3) particle size, polydispersible index & zeta potential was found to be 38.1nm, 2.88 & -4.49mV respectively. The optimized formulation was stable at 400C/75 % RH for 3 months. These formulations have displayed good Nanosuspension strength.

Keywords: Bosentan, oral Nanosuspension, Urea, SLS, Poloxamer (188), TWEEN-80, Ethyl acetate and Methanol.