Abstract

ANTIDIABETIC EFFECT OF BRIDELIA RETUSA SPRENG. BARK ON ALLOXAN-INDUCED DIABETIC RATS

Suhas Suresh Awati*, Sujit Arun Desai, Dhanraj Raghunath Jadge, Sunil Sambhaji Karale

ABSTRACT

Objectives: To evaluate the antidiabetic activity of ethyl acetate and methanolic bark extracts of Bridelia retusa Spreng. on alloxan-induced diabetic rats. Method: Diabetes was induced by a single dose of alloxan (120 mg kg-1 i.p) monohydrate. The acute and chronic study included the measurement of blood glucose level at 0, 1, 3, 5, 7, 24 hr and on 15th day after administration of extracts. Glibenclamide was used as a standard (5mg kg-1 orally). Result: Ethyl acetate and methanolic extracts showed more significant (P<0.01) reduction in blood glucose level when compared with diabetic control. Similarly showed significant decrease in total cholesterol, triglycerides, transaminase, ALP and urea level and increases the serum insulin level in alloxan induced diabetic rats. An oral glucose tolerance test showed significant improvement in glucose tolerance in the animals treated with ethyl acetate and methanolic extract. Histopathological estimation of pancrease showed regeneration of β-cells. The results obtained were comparable with reference standard drug glibenclamide. Conclusion: The present study concluded that the Bridelia retusa is an effective herb against alloxan-induced diabetes and also in preventing the metabolic alteration induced as the consequence of diabetes.

Keywords: Alloxan, Antidiabetic, Bridelia retusa Spreng, Insulin.