Abstract

DEVELOPMENT AND EVALUTION OF HEPATOPROTECTIVE HERBAL FORMULATION. (EXTRACT FRACTIONS)

Dr. K. Srisailam*, Kanakam Vijayabhaskar and N. L. Gowrishankar

ABSTRACT

Objective: The objective of the study is to separate and identify the most effective hapatoprotective fraction of methanolic extract of Picrorrhiza kurroa, Eclipta alba, Terminalia chebula, Tribulus terrestris, Piper nigrum and Nigella sativa by fractionating and evaluating its fractions for hapatoprotective activity in three mechanistically dev4ised models viz., CCl4, induced liver damage in rats. Liver damage was induced in different groups of rats by administering 1:1v/v CCl4 in olive oil 1ml/kg.b.w.p.o, the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters, histology of liver of rats and the most effective bioactive fraction was screened for its effect on hepatic microsomal drug metabolizing enzymes. Pretreatment with fractions (1-butanol, toluene, EA, at 50,100mg/kg.b.w) significantly reversed the changes in serum biochemical parameters and histology of liver caused by the three hepatotoxins namely CCl4, indicating their hepatoprotective activity. The hepatoprotective activity of butanol fraction of poly hebals was well supported in PT studies. Conclusion: All the fractions of poly herbal of methanolic extracts exhibited significant hepatoprotective activity and butanol fraction of poly herbs (100mg/kg) was identified as the most effective fraction.

Keywords: Hepatoprotective activity, CCl4, butanol, toluene, ethyl acetate, Silibinin.