Abstract

NON RECOMBINANT MUTAGENESIS OF BACILLUS CEREUS FOR FIBRINOLYTIC PROTEASE PRODUCTION

E. Venkata Naga Raju*, Goli. Divakar

ABSTRACT

The advent of protein engineering and sophisticated molecular technologies has opened possibilities for screening variants of enzymes and tailor-made proteins from microorganisms with enhanced production yields which may be of interest for specific commercial applications. This work has been undertaken to enhance the production of clinically important fibrinolytic protease by subjecting indigenous fibrinolytic protease producing Bacillus cereus to non-recombinant random mutagenesis using ultra-violet (UV) irradiation, ethyl methane sulfonate (EMS) and ethidium bromide treatment. Mutants were screened on the basis of enzyme assay by spectrophotometer using folin’s phenol reagent.

Keywords: non-recombinant, Bacillus cereus, fibrinolytic protease, folin’s phenol reagent.