Abstract

PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIOXIDANT ACTIVITY OF MEMECYLON UMBELLATUM ROOT EXTRACTS

Suresh Killedar*, Harinath More, Gourav Shah, Suryakant Gaikwad

ABSTRACT

The objective of this study to find out the DPPH scavenging (antioxidant) activity of dry extract of Memecylon umbellatum root obtained by various solvents such as petroleum ether, chloroform, ethyl acetate, acetone, methanol and chloroform water (IP). The invitro free radical scavenging activity was recorded by DPPH method using various concentrations of dry extract in distilled water (1, 2, 4, 8, 16, 20μg/ml) against blank with ascorbic acid as a standard in same concentrations. From among the extract the acetone extract showed maximum 95.770±0.042% antioxidant activity at 20μg/ml while petroleum ether shows minimum 5.290±0.0482%. The IC50 value of acetone extract was found to be 10.44±0.0846μg/ml as compare to standard ascorbic acid 3.76±0.0856μg/ml. By using Graphpad Instat 3 software paired t test of acetone root extract exhibits significant results (p<0.05) compared to standard ascorbic acid. In future it may found as a main source for dietary antioxidants and nutrient.

Keywords: Memecylon umbellatum, DPPH reagent, Antioxidant activity, IC50.