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Abstract

DETERMINATION OF ?max OF ANTIBIOTIC F-40 PRODUCED BY Streptomyces sulfonensis sp.nov.,

Ellaiah P., Sri Venkateswara Rao V.1*, Ramakrishna S. V., Venkateswarulu Y., Annapurna J. and Venkatesam U.

ABSTRACT

A total of 359 actinomycetes were isolated from 8 different natural substrates collected from different locations of Indian state of Andhra Pradesh. The taxonomic studies and literature survey indicates that the potent antibiotic producer was belongs to a new streptomycete species. The antibiotic F-40 was isolated from the culture filtrate of S.sulfonensis by extraction with organic solvents and it was purified and separated by TLC and column chromatography methods. As a part of its characterization, UV λ max was determined using dichloromethane as solvent. The λ max was found to be 232 nm. The data indicates the aromatic nature of the compound and it was used along with other physico-chemical properties in the identification of antibiotic F-40.

Keywords: Actinomycetes, Streptomycete, S.sulfonensis, chromatography, UV, ? max.


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