Abstract

IN VITRO SHOOT INDUCTION AND MULTIPLE SHOOT REGENERATION IN CISSUS QUDRANGULARIS L. (HADJOD) A MEDICINALLY IMPORTANT PLANT

Kalpana Teware*

ABSTRACT

Cissus qudrangularis (Hadjod) is an important medicinal plant used in India. It is used by the tribal and rural for bone healing purpose traditionally. We have not reported yet any standard protocol for in vitro propagation for this. Objective: to establish and optimize the rapid propagation technology of Cissus quadrangularis L. and to generate and try to enhance the phytochemical found in this. Material and method: we used different conc. of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) kinetin (KT) was used to establish and screen the optimal rapid propagation technology of Cissus qudrangularis. Results: by using apical bud and meristem we have found induction of root and multiplication on Murashige and Skoog’s medium (ms; 1962)supplemented with 3.5mg/l BAP and 0.5 NAA, in this combination +_3 shoot per plant were obtained Culture had been established successfully after a month in 2.5BAP + 1.5NAA. After this it transferred to the higher media BAP and NAA (3.5+ 0.5). We have found multiple shoot in this. Conclusion: obtained plant will be tried for hardening for rapid multiplication purpose.

Keywords: micro propagation, Cissus qudrangularis, shoots meristem, ms medium. NAA, BAP.