Abstract

PRODUCTION OF XYLANASE BY PENICILLIUM FREQUENTANS ISOLATED FROM LITTER SOIL USING PADDY STRAW AS SUBSTRATE BY SOLID STATE FERMENTATION

Thenmozhi V. Arulanandham and Muthusamy Palaniswamy*

ABSTRACT

Xylanase is one of the commercially and industrially important enzymes. Production of xylanase using, Penicillium frequentans, isolated from soil was studied. Penicillium frequentans isolated from litter soil was grown on paddy straw to produce xylanase using solid state fermentation. Various growth factors which influence the solid state fermentation were optimized to achieve maximum enzyme production. In the present study, Penicillium frequentans showed a higher enzyme production of 294 IU/mL when grown on paddy straw with an optimum temperature of 30oC, moisture content of 30%, 4 days of incubation, inoculum of 105 spores/mL and inoculation with 5 days old culture.

Keywords: Xylanase, solid state fermentation (SSF), agro-industrial wastes, paddy straw, optimisation.