Abstract

IDENTIFICATION AND EVALUATION OF ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF PENICILLIUM ISLANDICUM AND ASPERGILLUS TAMARII ETHYLE ACETATE EXTRACTS

Heba Abdel-Hady*, Marwa Tamim Ahmed Abdel-Wareth, Eman Ahmed El-Wakil,
Eman Ahmed Helmy

ABSTRACT

Fungi are known as important source of natural products. They are
prolific producers of secondary metabolites. This research was
undertaken to evaluate the antimicrobial and cytotoxic activity of ethyl
acetate extracts of Penicillium islandicum and Aspergillus tamarii
extracellular secondary metabolites. In this study, Fungi were
inoculated in potato dextrose broth medium then filtrates of both fungi
were extracted with ethyl acetate 1:1(v/v) and evaporated to obtain the
crude extract. The extract of each fungus was evaluated for its
antimicrobial activity against some fungi in comparison to
Amphotericin B and bacteria (gram-positive and gram-negative) in
comparison to Ampicillin and Gentamicin, respectively using diffusion agar technique. Also,
anticancer activity towards hepatocellular liver carcinoma (HepG2) cell line was estimated.
The results exhibited a strong anti-bacterial activity of both extracts and higher cytotoxic
effect on HepG2 cell line of Aspergillus tamarii extract than Penicillium islandicum. GC-MS
analysis of both extracts proved the presence of two major compounds in Aspergillus tamarii
are 1,2-Benzenedicarboxylic acid, mono(2-ethylhexyl) ester at Rt 31.33 (21.02%) and 1,2-
Benzenedicarboxylic acid, bis(2-methylpropyl) ester at Rt 19.76 (9.63%) and the major
compound in Penicillium islandicum is 1,2-Benzenedicarboxylic acid, mono(2-ethylhexyl) ester at Rt 31.39 (63.15%). These results revealed that both fungal extracts could be used in pharmaceutical industry as drugs for some infectious diseases and hepatocellular carcinoma.

Keywords: Aspergillus tamarii, Penicillium islandicum, GC-MS, Antimicrobial, Cytotoxic.