WJPPS Citation

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • New Impact Factor
  • WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

  • WJPPS: MARCH ISSUE PUBLISHED
  • March Issue has been successfully launched on 1 March 2024.

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • Scope Indexed
  • WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

Abstract

ANTIBACTERIAL EFFECTS OF AERIAL PART OF WITHANIA SOMNIFERA WITH CHLOROFORM EXTARCT ON PATHOGENIC STRAINS

Priyanka Kumari Panchal*, Nishi Sharma and Kamal Singh

ABSTRACT

The present study was planned to investigate the microbial inhibitory effects of Withania somnifera leaves. The preliminary successive solvent extraction and chemical test revealed the presence of secondary metabolites in various solvent extracts. Microbial inhibitory effects has provide us clue for further investigation. Chloroform extract was used for investigation of inhibition on five pathogenic bacterial strains and done by broth dilution methods with triplicate. Tetracycline used as positive control and double distilled water was used as Negative control. Statistical analysis was done by Mean ± Standard Deviation with range, in which n=5 and one way ANOVA done with SPSS 20.0, in which p values were considered significant at 95% confidence intervals (p<0.05). The antimicrobial activity best results against B. thruengenesis and C. diphtheria and average effect against S. typhi, C pneumonia and P. aeruginosa. The plant leaves have shown the presence of alkaloids, flavanoids, steroids and anthroquinone. As we calculate that Withania leaf chloroform extract may be diluted with 5 mg/ml to get 12.96 activity against B. thruengenesis and C. diphtheria and 10 mg/ml to get 6.48 activity against S.typhi, C.pneumoniae and P. aeruginosa. We found that Withania somnifera leaves chloroform extract shows the very best inhibitory effect against all tested pathogenic bacteria. But B. thruengenesis and C. diphtheria were very sensitive with Withania leaves. It has shown the presence of flavonoids, alkaloids, steroids and anthroquinone, which is responsible to show antimicrobial, immunomodulatory effect. Withania leaves have shown a high level of activity against tested pathogenic strains. It will be beneficial to investigate the active constitute present in the Withania somnifera leaves to increase the therapeutic uses.

Keywords: Withania somnifera, secondary metabolites, antimicrobial activity, chloroform, total activity, statistical analysis, Phytoconstituents.


[Download Article]     [Download Certifiate]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More