Abstract

ANTI-HYPERGLYCEMIC POTENTIAL OF METHANOLIC LEAF EXTRACT OF RHYNCHOSIA BEDDOMEI BAKER AGAINST ALLOXAN INDUCED DIABETES IN RATS

*A. Rajani and A. Jaswanth

ABSTRACT

The present study was conducted to evaluate the Anti-hyperglycemic activity of methanolic leaf extract of Rhynchosia beddomei Baker against alloxan induced diabetes in rats. The dried leaves of Rhynchosia beddomei Baker were subjected to extraction by continuous hot percolation using methanol as solvent and further evaluated for anti-diabetic screening. Dose selection was made on the basis of acute oral toxicity studies (4000mg/kg body weight) as per OECD and CPCSEA guidelines. Alloxan induced diabetic rat model was used for the evaluation of Anti-diabetic activity. Glibenclamide was used as the standard to compare the test results. Oral administration of the test extract in the dose of 200 and 400mg/kg for 21 days resulted in significant reduction in blood glucose levels At the end of the experiment, the animals were sacrificed and the pancreas and liver were sectioned for histopathology. The histopathological studies of the pancreas of diabetic animals revealed the degeneration of pancreatic islet cells, but with the restoration after treatment with the dose of the test extract. The liver showed no histopathological change. The implications of the result obtained in the present study provide the scientific rationale for the use of Rhynchosia beddomei Baker as anti-diabetic agent in the management of diabetes.

Keywords: Rhynchosia beddomei Baker, methanolic extract, Anti-Diabetic activity, Alloxan, Glibenclamide.