Abstract

MONO NUCLEOTIDES DETERMINES THE FREQUENCY OF UNSTABLE MICROSATELLITES BIOMARKERS IN PATIENTS WITH NON-HEREDITARY COLORECTAL CANCER POPULATION OF THE CITY OF TABRIZ IN IRAN

*Shahin Asadi and Parisa Ghahvechi

ABSTRACT

Short Tandem Repeats motifs DNA microsatellites instability in the accumulation of mutations in genes that occurs due to repair-deficient nucleotide pairing is done. So can be used as an alternative biomarker for assessing gene function in repairing the defect base pair in the colon is bad. Also, instability microsatellites not only a biomarker for colorectal cancer screening is hereditary, but in 15% of cases of nonhereditary colon cancers can also be evaluated. The aim of this study was to determine the frequency and evaluation 5 biomarker microsatellites monomorphic in patients with non-hereditary colon cancer in the city of Tabriz. Under the project 110 patients with nonhereditary colon cancer were evaluated in terms of instability microsatellites. In this study, a panel of five biomarkers multiplex Penta microsatellites monomorphic (BAT-25, NR-24, NR-27, NR-21, BAT-26) were used. The results showed that the most abundant biomarker biomarker NR-21 was unstable compared with other biomarkers. % 74/2 patients in the study were divided panel of five biomarkers were unstable Mono-nucleotide. The frequency instability of two biomarkers BAT-25 and NR-24% 17/8 was determined and the frequency of instability biomarkers BAT-26% 8/3 and NR-27% 1/7 respectively. According to the results, we can conclude that biomarkers NR-21, BAT-25, NR- 24 as a biomarker in the panel are unstable.

Keywords: colorectal cancer, instability of microsatellites, biomarkers mono-nucleotide.