Abstract

EXTRACTION OF ACETYLCHOLINE ESTERASE INHIBITORS FROM PLUMERIA PUDICA AND ANALYZING ITS ACTIVITY ON ZEBRAFISH BRAIN

W. W. Chanaka Prasad, M. P. Manuja Viraj, Roja Philip, Jyothi Rani, Swetha. BN. Guru Prasad R*

ABSTRACT

Alzheimer disease is neuro-degenerative disorder where acetylcholine esterace (AChE) plays key role, in the progression of the disease. The enzyme AChE plays a major role in the dissociation of the neurotransmitter acetylcholine (Ach) in to acetic acid and choline and there by affecting the nerve transmission. One of the current scenario for the treatment of Alzheimer disease is to inhibit the activity of AChE, which decrease the degree of neuro-degeneration. AChE inhibitors are found to be naturally present in the plants. Hence the methanolic extract of the leaves of Plumeria pudica was screened for AChE inhibitors for the brain homogenate of the zebrafish (Danio rerio). Through the titri-metric analysis of the AChE in in-vitro and invivo conditions the activity was recorded. We have found that the methanolic extract of Plumeria pudica reduces the activity of AChE. Under in-vitro and invivo conditions the activity of the AChE reduce from 15.8%-18%.

Keywords: Alzheimer disease, neuro-degenerative disorder, acetylcholine esterase, Plumeria pudica, Danio rerio[zebrafish].