Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF METHOCARBAMOL AND DICLOFENAC SODIUM IN INJECTION DOSAGE FORM

Nikita P. Patel* and Madhuri Hinge

ABSTRACT

A rapid, specific and economic UV spectrophotometric method has been developed using solvent composed of methanol: water (10:90) to determine the content of Methocarbamol and Diclofenac sodium in injection dosage form. Estimation of Methocarbamol and Diclofenac sodium was carried out at 274.11 nm (max. wavelength of MET) and 282.45 nm (isoabsorptive point) for Absorbance Ratio method and 223.92 nm for Diclofenac sodium (ZCP of Methocarbamol) and 281.69 nm for Methocarbamol (ZCP of Diclofenac sodium) for First order derivative spectroscopy method. Methocarbamol and Diclofenac sodium were found to be linear over the range of 12-60 μg/ml and 2-10 μg/ml respectively for both the methods. The method was validated statistically and studied for linearity, precision, accuracy, LOD and LOQ. The obtained results proved the method can be employed for the routine analysis of Methocarbamol and Diclofenac sodium in injection dosage form.

Keywords: Methocarbamol, Diclofenac sodium, Q Absorbance ratio method, First Order Derivative method.