ISOLATION, IDENTIFICATIONAND SCREENING OF EFFECTIVE CELLULASE PRODUCING FUNGAL STRAINS FROM AGRICULTURAL WASTE DUMPING AREA
R. Mangalanayaki* and S. Madhavan
ABSTRACT
The 10 different fungal strains were isolated from agricultural waste
dumping area in an around Thanjavur District. The effective cellulase
producing fungal strains were screen by using carboxymethylcellulose
(CMC) plates. After incubation the culture plates were flooded either
with 1% hexadecyltrimethyl ammonium bromide or with 0.1% Congo
red followed by 1 M NaCl. In both cases, it takes a minimum of 30 to
40 minutes to obtain the zone of hydrolysis after flooding, and the
hydrolyzed area was measured. Among the 10 fungal strains the
Trichoderma harzianum showed maximum zone of hydrolysis
followed by Aspergillus flavus, Aspergillus niger and Fusarium
oxysporum. These isolates were stored at 4°C and it will be used for
further studies into the enzyme production and their ability to degrade
cellulose.
Keywords: Cellulase, Trichoderma harzianum, Aspergillus niger, Fusarium oxysporum and carboxymethylcellulose.
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