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Abstract

FAST AND SENSITIVE HPLC METHOD FOR THE DETERMINATION OF ZOLPIDEM IN BULK AND FORMULATION

S.V.V. Dhanu Radha*, K.M. Ch. Appa Rao and K. Rama Krishna

ABSTRACT

A simple, rapid, accurate, precise method has been developed for the estimation of Zolpidem in bulk and formulation using reverse phase high pressure liquid chromatography. The method was developed on C18, HYPERSIL250 mm length, 4.6 mm inner diameter column in an isocratic mode using mobile phase containing a ternary mixture of ammonium acetate, methanol and acetonitrile. (30:45:25, v/v/v) The flow rate and detection wavelength were 1.0ml/min and 248nm respectively. The retention time of the drug was found to be 3.05min and the method produced was linear in the concentration range of 30-90 μg/ml.

Keywords: Benzodiazepines, Zolpidem, HPLC, Ammonium acetate and Acetonitrile.


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