Abstract

HISTOPATHOLOGICAL EVALUATION OF THE PANCREAS FOLLOWING ADMINISTRATION OF PARICALCITOL IN ALLOXAN- INDUCED DIABETIC WISTAR RATS

*El-Esawy BH, Alghamdy AN, El Askary A, Elsayed EM

ABSTRACT

Background: Type 2 Diabetes mellitus (T2DM) is a disorder that results from pancreatic insufficiency, insulin resistance or suboptimal responses to insulin mediated actions. Objectives: The current study investigated the effects of Vitamin D analog paricalcitol on pancreatic histopathology in T2DM. Methods: Twenty-four wistar rats weighing between 150 and 170g were used in this research work. They were divided into four groups A, B, C, and D of six rats each. Diabetes was induced in Groups B, C, and D using a single intra-peritoneal injection of 150mg/kg of Alloxan after an overnight fast. Group A served as the normal control while Group B served as the diabetic group and each received 0.5ml of normal saline daily. Groups C& D received 0.4 μg /kg/day and 0.8 μg/kg/day of the paricalcitol respectively through orogastric intubation. All the animals were given normal rat chow and water freely. The experiment lasted for 28 days. The animals were anaesthetized using chloroform inhalation and the peritoneum stripped open and the pancreas removed and prepared for histological observation using haematoxylin and eosin staining technique. Results: paricalcitol-treated rats presented an amelioration of inflammation and fibrosis in endocrine and exocrine pancreas. Conclusions: Histopathological evaluation showed more regenerative changes of pancreatic islet cell at a dose of 0.8 μg/kg/day.

Keywords: Pancreas, Paricalcitol, diabetes mellitus, Wistar rats.