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Abstract

THE ANTIBACTERIAL ACTIVITIES OF CROCUS SATIVUS AGAINST MASTITIS PATHOGENS AND ITS ANTIOXIDANT ACTIVITIES

Gulten Okmen*, Sukran Kardas, Duygu Bayrak, Ali Arslan and Haldun Cakar

ABSTRACT

Mastitis is a complex disease, which is defined as inflammation of parenchyma of mammary glands. Antibiotics are widely used in the treatment of mastitis. However, this widespread use of antibiotics causes both antibiotic residues in milks and antibiotic resistance developed in bacteria. The aim of this work was to investigate the antibacterial effects of Crocus sativus extracts against mastitis pathogens, and its other biological activities. The aqueous extract showed maximum inhibition zone against one bacteria (Coagulase- Negative Staphylococci- 32; CNS 32), and the zone was 11 mm. Two bacteria (CNS – 33 and 36) showed the lowest sensitivity to 3250 μg/mL /mL concentration for ethanol. In addition, the extracts were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free-radical for antioxidant activity. As a result, the ethanol extract observed a strong antioxidant activity (trolox equivalent: 2,4 mM). The extracts of C. sativus have antibacterial and antioxidant activities.

Keywords: Crocus, Mastitis, Antibacterial Activity, Antioxidant Activity.


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