ISOLATION AND CHARACTERIZATION OF RHIZOBIA FROM ROOT NODULES OF SOYBEAN (GLYCINE MAX L. MERR.) GROWN IN FERRALSOLS OF DAKNONG AND DAKLAK PROVINCE, VIETNAM
Prof. Dr. Cao Ngoc Diep* and Duong Ba So
ABSTRACT
Total of 44 isolates were isolated from nodules of soybean grown on
ferralsols of CuJut district (24 isolates) and Buonho town (20 isolates).
The microsymbionts causing nodules on the roots of soybean (Glycine
max L. Merr.) and 34/44 rhizobial isolates were analysed with 16S-23S
intergenic spacer and PCR-RFLP for repeated sequence RSα a 1195-bp
DNA fragment, indole acetic acid production and nitrogen fixing
activity. Almost isolates produced IAA but they had the different
nitrogen fixation capacity. All the strains were fast-growing soybean
rhizobia (Sinorhizobium [Ensifer) fredii except two isolates (CJ02 and
CJ09) were slow-growing soybean rhizobia (they were identified with
sequence RSα as molecular marker for Bradyrhizobium japonicum).
The study also showed the low presence of soybean nodulating slow
growers in ferralsols of western highland of Vietnam.
Keywords: Bradyrhizobium, ferralsols, Sinorhizobium, soybean, western highland of Vietnam.
[Download Article]
[Download Certifiate]