ANTI-DIABETIC AND ANTIOXIDANT ACTIVITY OF NOVEL DIHYDROXY GYMNEMIC TRIACETATE (DGT) IN LIVER OF HIGH FAT DIET AND FRUCTOSE-INDUCED TYPE-2 DIABETIC ADULT MALE RAT
Jayaraman Selvaraj*, Daisy Pitchai, P. Nithya, G. Valli, Rajagopal Ponnulakshmi, Govindan Ramajayam
ABSTRACT
Background: Dihydroxy gymnemic triacetate (DGT) is a novel phytocompound isolated from the leaves of Gymnema sylvestre. This compound has been shown to have anti-hyperglycaemic activity in streptozotocin-induced type-1 diabetic rats. However, the effect of DGT in high fat-diet induced insulin resistance which is one of the causative factors for the development of type-2 diabetes is obscure. Objectives: The present study was undertaken to evaluate the diabetic amelioration of DGT in liver of high-fat diet and fructose-induced type-2 diabetic adult male rat. Materials and Methods: Wistar male albino rats were divided into 5 groups. Group I: Control. Group II: Type-2 diabetic rats. Group III: Type-2 diabetic rats treated with DGT (20mg/kg body weight/day orally). Group IV: Type-2 diabetic ratstreated with metformin (50mg/kg body weight/day orally). Group V: Normal rats treated with DGT (20mg/kg body weight/day orally). After 45 days of treatment, fasting blood glucose, oral glucose tolerance, lipid peroxidation and reactive oxygen species, antioxidant enzymes, and the glycogen level in liver were assessed. Results: Diabetic rats showed impaired glucose tolerance, impairment in antioxidant enzymes and glycogen concentration. Lipid peroxidation (LPO) and free radical generation were increased in diabetic control rats. The treatment with DGT normalized the altered levels of blood glucose, LPO and free radical generation. Conclusion: Our present findings indicate that the novel DGT restored the high-fat diet and fructose-induced detrimental changes in glucose homeostasis by normalizing the blood glucose, LPO and free radical generation. Hence, DGT act as a potential anti-diabetic and antioxidant agent.
Keywords: Dihydroxy gymnemic triacetate, type-2 diabetes, high fat diet, liver.
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