Abstract

ASSESSMENT OF PATIENTS WITH MUSCULAR DYSTROPHY IN TABRIZ AT THE PROTEIN LEVEL USING TECHNIQUES OF IMMUNOHISTOCHEMICAL

Shahin Asadi*, Saeedeh Habibi, Ali Nazirzadeh, Javid Ghazizadeh

ABSTRACT

Muscular dystrophy often multisystem disease with progressive skeletal muscle weakness, defects in muscle proteins are Divided into 9 different categories of these cases can be, Dystrophynopaty the scapula and pelvic muscular dystrophy, congenital muscular dystrophy noted. Diagnosis using clinical and laboratory techniques, such as measuring the enzyme activity of CK, ultrasonography, or electromyography and molecular tests PCR and muscle biopsy to be used in genetic counseling, treatment for possible future use, prenatal diagnosis and reduction of muscular dystrophy, the level needed. So for the first time in Tabriz, Iran for the detection and differentiation of muscular dystrophy, muscle biopsies on 89 patients at the protein level Histopathology and immunohistochemistry was performed, followed by the study. A total of 58 patients were identified, of which 37 patients Dystrophynopaty methods included 11 patients with Duchenne muscular dystrophy and Becker muscular dystrophy patients under 26, 21 patients with muscular dystrophy Limb - Girdle Sarcoglycanopathy 17 patients and 4 patients with absence Merosin Dyspherlynopaty and 9 patients (congenital muscular dystrophy), respectively.

Keywords: muscular dystrophy, identify the level of protein synthesis, immunohistochemical techniques.