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Abstract

SIMULTANEOUS ESTIMATION OF PIOGLITAZONE AND GLIMEPIRIDE IN BULK AND PHARMACEUTICAL FORMULATION BY RP-HPLC METHOD

K. Vamsee Krishna Reddy*, R. Madhusudhan Raju, K. Rajasekhar reddy

ABSTRACT

A simple, rapid, precise and accurate reverse-phase HPLC method was developed and validated for the simultaneous determination of Pioglitazone HCL and Glimepride in commercial tablets. The method has shown adequate separation for Pioglitazone HCL and Glimepride. Separation was achieved on YMCProYMC Pack ProYMC Pack ProYMC Pack ProYMC Pack ProYMC Pack ProYMC Pack ProYMC Pack Pro C18 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 (250 mm × 4.6mm; 5 µm) columnµm) columnµm) columnµm) columnµm) columnµm) columnµm) columnµm) columnµm) columnµm) column using isocratic method with the flow rate 1.0ml/min and 0.1 M CH3COONH4 :Methanol(60:40) system at 30oC column temperature and the detection was carried out at 248nm using photodiode array (PDA) detector. The linearity of the proposed method was investigated in the range of 54-162μg/ml (r2=0.999), 7.2-21.60μg/ml (r2=0.999) for Pioglitazone HCL and Glimepride Respectively. The limit of detection (LOD) was 0.149 and 0.0133 for Pioglitazone HCL and Glimepride respectively. The limit of quantification (LOQ) was 0.496 and 0.0442 for Pioglitazone HCL and Glimepride respectively. The relative standard deviation (RSD) of five replicates is less than 2%. This HPLC method is applied successfully to the simultaneous quantitative analysis of Pioglitazone HCL and Glimepride in commercial tablets.

Keywords: RP-HPLC, Pioglitazone HCL ,Glimepride,Simultaneous Estimation


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