Abstract

HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY- A MODERN ANALYTICAL SEPARATION TECHNIQUE FOR NATURAL PRODUCTS

*Vaibhav Srivastava¹, Subodh Dubey², Shyam B. Sharma³

¹Nagaji Institute of Pharmaceutical Science, Gwalior (M.P.) India.
²IPS College of Pharmacy, Gwalior (M.P.) India.
³SOS in Pharmaceutical Science, Jiwaji University, Gwalior (M.P.) India.

ABSTRACT

Standardized manufacturing procedures and suitable analytical tools are required to establish the necessary framework for quality control in herbals. The separation techniques including “High Performance Liquid Chromatography”, “High Performance Thin Layer Chromatography” and “Capillary Electrophoresis” are most widely used to establish reference “finger prints” of herbs which is one of the most powerful tools to link the botanical identity to the chemical constituent profile of the plant. HPTLC technique applied for compilation of profiles pertaining to varied range of bioconstituents such as alkaloids, glycosides, terpenoids, flavanoids, saponins, resins, coumarins, plant hormones, antibiotics and number of other compounds of natural origin. From the constituent profile, a number of marker compounds can be chosen which might be used to further describe the quality of the herb or herbal preparation. HPTLC can also be employed for quantitative determination of such marker compounds. High Performance TLC plates become commercially available since mid-1970s.followed by improvement in instrumentation. HPTLC involves- optimized coating materials, improved dosage (spotting) method, competent data acquisition and processing system. Hence HPTLC is very useful in qualitative and quantitative analysis of herbals and fit in practical aspects, as it combines the art of chromatography with quickness at a moderate cost.

Keywords: HPTLC, alkaloids, glycosides, terpenoids, flavanoids, saponins.