Abstract

DETECTION OF CYTOMEGALOVIRUS IN IRAQI RECURRENT MISCARRIAGE WOMEN

Marwah Yousif Hameed* and Ismail Hussein Aziz

ABSTRACT

This study was aimed to investigate the existence of HCMV in recurrent miscarriage women by using Real- time PCR and compare it with enzyme-linked immunosorbent assay (ELISA). Seventy blood samples were examined from recurrent miscarriage women and forty from women with normal pregnant (aged between 18 and 45 years old) which the patients were suspected to be infected with HCMV. Serological technique included Enzyme-linked Immune sorbent Assay (ELISA) was done to detect the presence of anti - Human Cytomegalovirus antibody IgG and IgM against the virus in the patient’s serum. The serology test showed that all samples of recurrent miscarriage women 70(100%) seropositive to IgG and 28(40%) seropositive to IgM. Compared with control women 35 out of 40seropositive to IgG and no positive results to IgM. Molecular analysis using Real-time PCR for detection the presence of HCMV DNA in the samples. The results for Real-time PCR showed that ; 16 out of 70 (22.85%) patients were found to be HCMV positive while 54 out of 70 (45.65%) patients were found HCMV negative. According to the results of this study, Real Time – PCR technique was used for the detection of Human Cytomegalovirus in whole blood specimens had high sensitivity, effective and more specificity than serological methods ( ELISA).

Keywords: CMV; ELISA; PCR.