Abstract

ANDROGRAPHIS PANICULATA LEAF EXTRACTS AS POTENTIAL NAJA NAJA ANTI-SNAKE VENOM

Sivakumar A*, Manikandan A, Rajini Raja M and Jayaraman G

ABSTRACT

Traditional medicinal plants from the local region of Javvadu hills, Tamil Nadu, India were used as an antidote for snake bites by irrula tribes. One such a plant Andrographis paniculata leaves were collected from the traditional healers and explored for the for anti-snake venom activity. It was observed that the methanolic leaf extract of Andrographis paniculata significantly neutralized the Naja naja venom in vitro. The leaf extract of Andrographis paniculata was used to evaluate the enzyme inhibiting activity of protease, phosphomonoesterase, phosphodiesterase, acetyl cholinesterase, phospholipase A2, hyaluronidase and L-amino acid oxidase toxic enzymes present in snake venom. In-silico results of individual plant metabolites inhibiting PLA2 correlates with the results of plant extract inhibiting enzymes.

Keywords: Andrographis paniculata, Naja naja, acetyl cholinesterase, phospholipase A2.