HPTLC ANALYSIS OF TANNIN PROFILE IN THE LEAF AND BARK SAMPLES OF LORANTHUS LONGIFLORUS DESR COLLECTED FROM TWO HOST TREES.
L. Chandrakasan and R. Neelamegam*
ABSTRACT
Effect of host plants on the Tannin profile of Loranthus longiflorus leaf and bark samples collected from Casuarina equisetifolia and Ficus religiosa host trees were determined by HPTLC method. The methanol extract of L. longiflorus leaf samples obtained from C. equisetifolia and F. religiosa host trees showed 13 and 6 compounds, respectively, and were compared with gallic acid standard. Among the compounds, 6 and 4 compounds, in each sample, respectively, was identified as tannins while other compounds were unknown. Two compounds from each L. longiflorus leaf samples collected from C. equisetifolia (peak no. 3 & 6) and F. religiosa (peak no. 1 & 2) host trees showed similar Rf values (0.27 & 0.43, respectively). On the other hand, the methanol extract of L. longiflorus bark sample collected from C. equisetifolia and F. religiosa host trees contained 10 and 9 compounds in each
sample, respectively, and were compared with gallic acid standard. Among the compounds, 1 and 2 compound was identified as tannins in the bark sample of L. longiflorus collected from C. equisetifolia and F. religiosa, respectively, while all other compounds were unknown and 1 compound from both bark samples obtained from C. equisetifolia (peak no. 1) and F. religiosa (peak no. 1) showing similar Rf values (0.12). One compound of L. longiflorus leaf (peak no. 12)/bark (peak no. 9) samples, from C. equisetifolia showing similar Rf value (0.92) and one in the leaf (peak no. 1) and park (peak no. 3) samples of L. longiflorus collected from F. religiosa showing similar Rf value (0.27).
Keywords: Hemiparasite, Loranthus longiflorus, Leaf/bark methanol extracts, HPTLC analysis, Tannin, Casuarina equisetifolia host, Ficus religiosa host.
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