Abstract

METHOD DEVELOPMENT AND VALIDATION FOR IDENTIFICATION OF 2-PHENYL PHENOL IN SIAPTON 10L BY GAS CHROMATOGRAPHY MASS SPECTROMETRY

Sudeep Mishra, Mahesh Kr. Saini, Mukesh Kr. Singh, Samsul Alam, S. K. Raza, L. K. Thakur*, Shibendu Mukharjee

ABSTRACT

Method was developed and validated for identification of 2-Phenyl Phenol by Gas Chromatography Mass Spectrometry in Siapton 10 L. The retention time of 2- Phenyl phenol was approximately 7.14 min with good peak shape. Instrument was linear for 2-phenyl phenol in concentration range from 0.05 – 1 mg/Kg, with correlation coefficient (r2) equals to 0.9993. The precision (%R.S.D.) at concentrations- 2 ppm, 1 ppm, 0.5 ppm, 0.2 ppm, 0.1 ppm, 0.05 ppm and 0.01 ppm was found to be 2.37, 1.12, 1.31, 3.19 and 2.99 respectively which shows good precision with S/N ratio 3.5:1 of the developed method. LOQ and LOD concentrations 0.01 mg/Kg, 0.05 mg/Kg respectively confirm good sensitivity of the method. The developed GC method with Mass detector was accurate, precise, reproducible and sensitive. All the validation parameters of the method show good result

Keywords: 2-Phenyl Phenol, DCM, LOQ & LOD, SIM & SCAN, GCMS.