DETERMINATION OF PHENYL PROPANOIDS PROFILE IN THE LEAF AND BARK SAMPLES OF LORANTHUS LONGIFLORUS DESR COLLECTED FROM TWO HOST TREES BY HPTLC.
L. Chandrakasan and R. Neelamegam*
ABSTRACT
Influence of host plants on the phenyl propanoids profile of Loranthus longiflorus leaf and bark samples collected from Casuarina equisetifolia and Ficus religiosa host trees were determined by HPTLC method. The methanol extract of L. longiflorus leaf samples obtained from C. equisetifolia and F. religiosa host trees showed 6 and 7 compounds, respectively and were compared with eugenol standard. Among the compounds, 5 and 6 compounds in each sample, respectively, was identified as phenyl propanoids while the remaining one unknown. Two compounds from each L. longiflorus leaf samples collected from C. equisetifolia (peak no. 2 & 3) and F. religiosa (peak no. 2 & 3) host trees showed similar Rf values (0.16 & 0.27, respectively). On the other hand, the methanol extract of L. longiflorus bark sample collected from C. equisetifolia and F. religiosa host trees
contained 8 and 6 compounds in each sample, respectively and were compared with eugenol standard. Among the compounds, 5 and 3 compounds, in each sample, was identified as phenyl propanoids while other compounds were unknown and 2 compounds from both bark samples obtained from C. equisetifolia (peak no. 2 & 6) and F. religiosa (peak no. 1 & 4) showed similar Rf values (0.21 & 0.62). None of the compounds from leaf/bark samples of L. longiflorus collected from C. equisetifolia showing similar Rf values while one compound (peak no. 7 & 6) of L. longiflorus leaf/bark samples, respectively, from F. religiosa showing similar Rf value (0.94). The results of present study indicate that the HPTLC analysis of methanol extracts of L. longiflorus leaf and bark samples from C. equisetifolia and F. religiosa host trees make certain the presence of phenyl propanoid compounds and the host trees influenced on the nature and number of phenyl propanoids present in the hemiparasitic plants.
Keywords: Hemiparasite, Loranthus longiflorus, Leaf/bark methanol extracts, HPTLC analysis, Phenyl propanoids, Casuarina equisetifolia host, Ficus religiosa host.
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