OPTIMIZATION OF CULTURAL CONDITIONS FOR MAXIMUM FRUCTOOLIGOSACHARIDE PRODUCTION BY BACILLUS SPECIES
Belorkar Seema*, Kaladhar DSVGK and Patel Yashwant
ABSTRACT
Fructooligosaccharides (FOS) are oligomers of fructose exhibiting the prebiotic properties. The FOS are found naturally in products like honey, onion etc. Plants and microbes have the potential to synthesize FOS by expressing an enzyme Fructosyltransferase (Ftase). The present investigation of production of FOS by indegineously isolated Bacillus sp from soil sample. The bacteria produced 10.02% total FOS after 12h of incubation time. The optimum temperature and pH were 600C and 6.00 respectively. The total FOS production increased to 29.60 % after optimization.
Keywords: Fructooligosaccharides (FOS), Fructosyltransferase.
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