Abstract

DETERMINE PHOSPHOLIPASE AND ASPARTIC PROTEINASES ACTIVITY AS VIRULENCE FACTORS IN CANDIDA SPECIES ISOLATED FROM VAGINAL INFECTION IN KIRKUK CITY

M.Sc. Bari Lateef Mohammed*, Pro. Dr. Kalil I. Bander, Dr. Thekra A. Hamad

ABSTRACT

Vaginal candidiasis is a common complaint among women of different age groups in any society whether or not they are sexually active. Although it is both treatable and mild, when left untreated, The study was set to detect Candida organisms in women vaginal. A prospective study of women vaginal swabs collected from Gynecological Clinic of Azadi teaching Hospital and some privet clinical Kirkuk, Iraq, and a total of two hundred (200) women swab were analysed for microscopy and culture, May 2014 and April 2015. Data on epidemiologic indices were collected from the patients, using structured interviewer- administered questionnaires. fifty- seven positive cultures were obtained, constituting 28.5% (n = 57) in a total of 200 women vaginal samples . A total of 28.5% ( n= 57) isolates were detected which comprises four different Candida species, namely Candida albicans, C. glabrata, C. krusei and C. kefyre respectively as well as isolated Cryptococcus lorynti with frequency of occurrence of 23%, 2%, 1.5%, o.5% and 1.5% respectively. The extracellular phospholipases and aspartic proteinase of Candida species are considered to play a significant role in the pathogenesis of human infections. Therefore eighteen clinical isolates of Candida species from human in vaginal infection of this study was to evaluate enzymatic activities in vitro were screened for phospholipase production in vitro (using an egg-yolk-agar medium).and aspartic proteinase vitro (using an bovine serum albumin BSA). eighteen isolates were tested by the phospholipase activity from the vaginal samples were C. albicans (10), C. glabrata (4), C. kefyr (3),C. krusei (1). The isolates tested demonstrated low varying degrees of phospholipase activity (Pz value: 4.60-20.00) . In this study showed Phospholipase activity was detected in all of isolates and only minor differences between isolates, The highest enzyme activity detected of C. glabrata (15.65) then C. albicans (11.45) , C. krusei (12.00) and the lowest enzyme activity detected of C. kefyr ( 11) According to these results, there was no statistically significant difference in the mean values of the phospholipase activities of the Candida species (p ≤ 0.05) . and the isolates tested demonstrated high varying degrees of Aspartic proteinase activity (Pz value: 0.000000-24.00) . In this study showed Aspartic proteinase activity was not detected in all of isolates, The highest enzyme activity detected of C. albicans (12.64) then C. glabrata (3.50) , the lowest C. krusei (1.33) and the absence enzyme activity detected of C. kefyr ( 0) According to these results, there was statistically significant difference in the mean values of the Aspartic proteinase activities of the Candida species (P ≤ 0.05).

Keywords: Candida species, namely Candida albicans, C. glabrata, C. krusei and C. kefyre.