Abstract

A PRELIMINARY STUDY TO SEE THE EFFECTS OF DIFFERENT CONCENTRATION OF GROWTH REGULATORS ON COMMON TRADITIONAL HERB

Dutta Amit Kumar*, Singh Monika and Bhattiwala Sakina

ABSTRACT

A Common Traditional Herb in Chhattisgarh like Stevia rebaudiana is a plant species in the genus Stevia of the sunflower family (Asteraceae), commonly known as sweet leaf, sugar leaf. Stevia is a tender perennial native to parts of Brazil and Paraguay that favours humid, wet environments, although the root does not tolerate standing water. Stevia is widely grown for its sweet leaves, which are the source of sweetener products known generically as Stevia and sold under various trade names. The active compounds are various Steviol glycosides (Stevioside and Rebaudioside), which have 250–300 times the sweetness of sugar. In my Tissue Culture Research protocols for shoot initiation and mass multiplication of Stevia rebaudiana L. have successfully been standardized and changes according to the my Lab conditions.The fundamental research was carried out on Grow Tips Biotech, Bhopal and the data was compiled at my present business place School of Biological & Chemical Sciences, Raipur on the basis of safety evaluation released by the World Health Organization found no adverse effects. Among these concerns are control of blood sugar and effects on the reproductive, cardiovascular, and renal systems. From my study, it was finally screened that in Stevia tissue culture, Shoot initiation was observed on MS medium with the different concentrations of hormone (Kinetin). The best results of initiation were obtained in MS medium with 0.05 mg/L Kinetin and in multiplication stage the development that we obtained during the initiation and establishment phase continued and it was observed that MS media with 1.0 mg /L Kinetin give best results for number of shoots and for explants length.

Keywords: Herb, MS Media, Tissue Engineering, Growth Regulator, Observation, Data Analysis (SPSS).