Abstract

PHYTOCHEMICALS STUDY, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF HELIANTHEMUM LIPPII (L.) PERS. IN DIFFERENT STAGES OF GROWTH (SOMATIC, FLOWERING AND FRUITING).

Chouikh Atef*, Feriani Anouar, Adjal El Hadda, Chefrour Azzedine

ABSTRACT

Objective: The aims of this work were to study the differences in amount of phenolics compounds and to estimate the antioxidant, antimicrobial activities of Helianthemum lippii (L.) with respect to growth stages (Somatic, Flowering and Fruiting). Methods: Three methanol extracts of different growth stages were prepared to estimation of phytochemicals content and evaluate the antioxidant and antimicrobial activities. Results: Yields of E.MOH extract is low during fruiting (6.5%) compared with the flowering period (8.97%) and the Somatic period (8.24%). The quantitative estimation of polyphenols and flavonoids showed that the extracts are rich in these compounds. Antioxidant activities showed that the methanol extract of the Somatic stage has a significant efficacy on DPPH radicals and hydroxyl, with the extract against methanol fruiting looks best on the free radical peroxide. As regards the extract from the flowering stage, it has been less significant results on all three radicals. The bacterial strains showed a very significant sensitivity via-a-via the extract of the flowering stage followed by fruiting, while a net resistance is observed with the extract of the Somatic stage. Conclusions: one can deduce that the introduction of this pastoral plant in the medical field would be desirable to enhance the therapeutic arsenal in the medical world.

Keywords: Helianthemum lippii (L.) Pers., Methanol extracts, Phytochemicals, Antioxidant activity, Antimicrobial activity, Stages of growth (Somatic, Flowering and Fruiting).