Abstract

EFFECT OF PLANT GROWTH REGULATOR ON THE IN VITRO MICROPROPAGATION OF BRYOPHILLUM TUBIFLORUM

Charan Singh*, Nevadita Sharma, Ajay Sharma, Bhawna

ABSTRACT

In the present study, we report a highly efficient and easy protocol for shooting and rooting process for using a bud of Bryophillum tubiflorum as explants. Different combination and concentration of cytokines and auxins are used in MS media as a supplement. Therefore, two combinations are 6-Benzylaminopurine (BAP), Kinetin, 1- Naphthaleneacetic acid (NAA); and; BAP, Indole-3- acetic acid (IAA) and Indole-3- butyric acid (IBA) are used for highest number of shoots and roots proliferation respectively whereas, these combinations show excellent results in 2.5 mg/l BAP + 0.5 mg. / NAA, 3 mg./l kinetin + 0.5 mg./l NAA; and; 0.5 mg/l BAP + 0.5 mg/l IAA, 0.5 mg/l BAP + 2.5 mg/l IBA respectively concentrations for shooting and rooting development. Thereafter, the In vitro regenerated plantlets were successfully acclimatized and established in soil with a survival of 88%.

Keywords: growth regulator, cytokinins, auxin, shooting, rooting.