Abstract

EFFECTS OF GLYCOSMIS PENTAPHYLLA LEAF EXTRACT AGAINST CHRONIC ARSENICOSIS IN RATS

Amartya De*, Akhilesh Mishra, S.K. Bandyopadhyay, T.K. Mandal, A.K.Das, M.C. Banerjee

ABSTRACT

The present study was conducted to evaluate the effects of methanolic extract of Glycosmis pentaphylla (GP) against sodium arsenite (NaAsO2) induced toxicosis in adult albino rats. Forty eight albino rats having body weight 150-200 gm of either sex were randomly divided into four groups viz., G0, G1, G2 and G3 each containing twelve. Sodium arsenite was administered at 4mg/kg b.w. daily in drinking water for 90 days to all rats of groups G1, G2 and G3. The rats of Group G2 and G3 were orally treated with leaf extract of GP at 320 mg/kg (1/10th of LD50) and at 160 mg/kg (1/20th of LD50) daily from 91st day to 120th day. Only distilled water was given to rats of G0 and considered as control. Blood samples were collected at different days for analysis
of haemogram, biochemical parameters like ALT, AST, BUN and CRT. Tissue samples were collected to study activity of SOD, MDA, GSH, Catalase and histopathology was conducted after completion of experiment. The results reveal that extraction of G.pentaphylla leaves may have ameliorative effect in arsenicosis in rats at dose dependent manner.

Keywords: Arsenic, Glycosmis pentaphylla, Haemogram , Histopathology, Rats.