Abstract

MICROPROPAGATION OF SOLANUM TRILOBATUM FROM SHOOT TIP EXPLANTS

Dr. H. David Raja*, K. Senthilarasu and Dr. D.I. Arockiasamy

ABSTRACT

Solanum trilobatum is a medicinally important perennial herb of the family Solanaceae. An efficient micropropagation protocol was established for the regeneration of Solanum trilobatum using shoot tip explants. Maximum multiple shoots (12 shoots/explant and 80% of response) were obtained from the shoot tip culture on MS medium supplemented with BAP (2.5mg/l). The shoots were rooted on MS basal medium supplemented with IBA (3.0mg/l). In vitro plantlets were transferred to pots containing a mixture of vermiculite and red soil (1: 1) for acclimation for a period of three - four weeks. The well-acclimatized plantlets were transferred to the field condition. The transplantation survival rate was 85 – 90% after 30 days.

Keywords: Micropropagation, Solanum trilobatum, Shoot tip, Multiple shoots.