Abstract

BIOCOMPATIBLE POLYMER GUM ACACIA COATED BOVINE SERUM ALBUMIN (BSA) NANOPARTICLES INCORPORATED AZITHROMYCIN (P-BSA NP-AZ) PREPARATION FOR THE IMPROVED ANTI BACTERIAL ACTIVITY AGAINST HUMAN PATHOGENIC BACTERIA

Department of Biotechnology, Sathyabama University, Chennai, Tamil Nadu, India.

ABSTRACT

Improved anti bacterial activity of biocompatible polymer coated bovine serum albumin nanoparticles incorporated azithromycin was evaluated against human pathogenic bacteria. Optimization of f BSA nanoparticles was studied with various parameters such as pH, Ethanol to BSA ratio and crosslinking time. Anti bacterial study was carried out with the nano conjugate against clinical isolate of human pathogenic bacteria Pseudomonas aeruginosa and Staphylococcus aureus adopting well diffusion assay. Determination of minimum inhibitory concentration was studied by broth dilution method.The optimal pH was found to be 8.0, the ethanol to albumin ratio was found to be 4:1 and cross linking time of 8hrs which gives the higher yield of BSA nanoparticles..The Nanospheres thus formed were characterized using scanning electron microscope which showed a particle size of nanosphere in the range of 145nm. Anti bacterial study was carried out with the nano conjugate against clinical isolate of human pathogenic bacteria,Pseudomonas aeruginosa and Staphylococcus aureus. All the tested bacteria were susceptible to the tested concentration of nano drug conjugate and the anti bacterial activity was increased with minimum 10 to maximum 20mm of zone of inhibition.Dsistinct increase in anti bacterial activity of polymer coated BSA nano drug conjugate against human pathogenic bacteria would suggests the possible utilization of polymer coated BSA nanoparticles incorporated azithromycin against pathogenic bacteria.

Keywords: bovine serum albumin nanoparticles, azithromycin,gum acacia, pathogenic bacteria,nano drug conjugate,improved activity.