Abstract

EVALUATION OF ANTIOXIDANT, ANTINOCICEPTIVE EFFECT OF CAMELLIA SINENSIS WITH COMBINATION OF GLIBENCLAMIDE IN DIABETIC NEUROPATHY RATS

L.Kinnera Kalagara*¹, Prem Kumar.N¹, Basavraj Sonnad¹

¹Department of Pharmacology, Krupanidhi College of Pharmacy, Bangalore -35, India.

ABSTRACT

In present study, we have made to attenuate diabetic neuropathic pain by implementing single and combined therapy of camellia sinensis extract (CSet) and Glibenclamide (Gli). Diabetes was induced by a single intraperitoneal injection of 35mg/kg streptozotocin. Diabetic rats were treated with Glibenclamide (4mg/kg), camellia sinensis (300mg/kg) and its combination. Oral glucose tolerance test was performed on every fifteenth day of treatment and there was a significant improvement in the glucose tolerance in the groups treated with glibenclamide, camellia sinensis and its combination with glibenclamide. Eddy’s hot plate, tail immersion (hot water) was used as diabetic neuropathic pain models, at the end of the treatment period, Thiobarbituric acid reactive substances (TBARS), Superoxide dismutase (SOD), catalase and histopathological examination of sciatic nerve were estimated to assess the oxidative stress. Preventive (8 weeks) and curative (4 week) treatment of camellia sinensis and its combination with glibenclamide significantly restored all the parameters and reduction in the sensitivity towards the pain in diabetic rats. This study provides experimental evidence to preventive and curative effect of camellia sinensis as antioxidant and antinociceptive in animal model of diabetic neuropathy.

Keywords: Diabetic peripheral neuropathy, camellia sinensis, glibenclamide, streptozotocin.