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Abstract

GENERATION AND DYNAMICS OF ANTIVENOM FROM CHICKEN EGG YOLK AGAINST SCORPION BITE

Ankit Choraria*, Maanoj.N.S, Lavanya.K and Michael.A

ABSTRACT

Mesobuthus tamulus also known as the Indian Red Scorpion is responsible for most of scorpion stings related to human incidents in southern parts of India. The only treatment for scorpion envenomation is immunotherapy based on administration of scorpion anti-venom produced in horses. Avian antibodies (IgY) isolated from chicken egg yolks represent a new alternative to be applied as anti-venom therapy. For this reason, we produced IgY antibodies against M. tamulus scorpion venom. The anti-scorpion venom antibodies were purified by precipitation techniques with polyethylene glycol and evaluated through indirect ELISA. Gas chromatography Mass Spectrometry was carried out and the compound present in the highest range was noted in a least sample of 2μl concentrations.

Keywords: Mesobuthus tamulus, Anti-venom, IgY antibodies, avian antibodies.


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