Abstract

PRODUCTION OF BIOSURFACTANT FROM BACILLUS MEGATERIUM AND PSEUDOMONAS FLUORESCENES ISOLATED FROM OIL CONTAMINATED SOURCES AND EXPRESSION OF BIOSURFACTANT PRODUCING GENES

*¹Amit Pandey and ²Ayush Saxena

ABSTRACT

The present study is carried out by isolation and characterization of biosurfactant producing microbes from oil contaminated sites. Bioremediation refers the clean up the environment using microbes. In this work the Bacillus megaterium and Pseudomonas fluorescenes were isolated according to Bergey’s manual and further used for oil degradation to check the production of biosurfactant. The Shake flask method, Agar well diffusion method and Spread plate method were used for oil degradation.Further the optimization was carried out for best carbon ,nitrogen sources and also for pH and temperature. And in last the pasmid was isolated from production media and after restriction digestion , ligation the transformation was done to transform oil degrading genes in to non oil degrading microbes.

Keywords: Bioremediation, Shake flask method, Agar well diffusion method, Spread plate method and optimization.