Abstract

ANTIOXIDANT STATUS OF METHANOLIC EXTRACT OF BRASSICA RAPA CHINENSIS LEAVES USING MAMMALIAN LIVER SLICE TECHNIQUE (IN VIVO SIMULATED IN VITRO MODEL)

Dharani Mayilsamy*. and Dr. Kalaivani Krishnaswamy

ABSTRACT

The influence of the methanolic extract of Brassica rapa chinensis (MEBRC) leaves on the enzymic and non- enzymic antioxidant activity in the liver tissue. The model was used an in vitro model which was very carefully designed to stimulate in vivo intraperitonial exposure of oxidant. This study was formulated with the recommendations laid down by FRAME (Fund for the Replacement of Animals in Medical Experimentation) to minimize the use of live animals for experimenting and to develop a model system that would stimulate in vivo conditions. The goat liver was selected as the mammalian tissue to determine the antioxidant effect of methanolic extract in the presence and absence of the standard oxidizing compound (H2O2) and the dose of H2O2 used was the same as the level used in vivo studies by intraperitonial administration (5μl/250mg tissue).The enzymic antioxidants assay such as superoxide dismutase (SOD), catalase(CAT), glutathione peroxidase(GPx), Gutathione-S-transferace (GST), Glutathione reductase (GR) and Glucose- 6-phosphate dehydrogenase (G6PD). The non- enzymic antioxidants determined were total reduced glutathione (TRG/GSH), vitamin-C and vitamin-E. Lipid peroxidation (LPO) was also measured in terms of MDA formed.

Keywords: Goat liver slice, Antioxidants, Brassica rapa chinensis, Bok choy.