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Abstract

UV SPECTROPHOTOMETRIC ESTIMATION OF DIACEREIN IN API AND CAPSULE DOSAGE FORM USING AREA UNDER CURVE METHOD

Dr. Hemant Kumar Jain* and Ravi S. Kadam

ABSTRACT

The aim of present work was to develop an accurate, precise, reproducible and economical UV spectrophotometric Area Under Curve method for estimation ofDiacerein. The Area Under Curve of UV spectrum was calculated between 246 to 266 nm and a simple method was developed.The method has followed linearity in the range of 2-10μg/ml.The value of correlation coefficient was 0.9976. This method was validated as per ICH guideline Q2 (R1).Satisfactory values of Percent relative standard deviation for the intra-day and interday precision indicated that method is precise. Results of the recovery studies (99.73% to 101.99%) showed accuracy of the method. LOD and LOQ were calculated as 0.70μg/ml and 2.13μg/ml,respectively. The developed method can be used for routine estimation of Diacerein in bulk and Capsule dosage forms.

Keywords: Diacerein, Area under Curve, UV spectrophotometry, Validation.


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