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Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF CURCUMIN IN BULK AND PHARMACEUTICAL FORMULATION

Vishal Vasant Holkar*, Deepika R. Nirmal and Sonali-Kapse Mistry

ABSTRACT

A simple, accurate, precise and sensitive UV Spectrophotometric method was developed for the determination of Curcumin in bulk and pharmaceutical dosage form. The solvent used is methanol and the wavelength corresponding to maximum absorbance of the drug was found at 421nm. Beers law was observed in the concentration range of 1-6μg/ml with correlation coefficient 0.999. The linear regression equation obtained by least square regression method were Y=0.015x+0.005, where y is the absorbance and x is the concentration of the pure drug solution. The method was validated for several parameters like accuracy, precision as per ICH guidelines. The values of relative standard deviation and % recovery were found to be satisfactory, indicating that the proposed method is precise and accurate and hence can be used for the routine analysis of Curcumin in bulk and pharmaceutical formulation.

Keywords: Curcumin, UV spectrophotometry, ICH.


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